CENTRE ON THE PROBLEMS OF ECOLOGY AND PRODUCTIVITY OF FORESTS
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CENTRE ON THE PROBLEMS OF ECOLOGY AND PRODUCTIVITY OF FORESTS |
RUSSIAN ACADEMY OF SCIENCES |
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I. N. Tret'yakova, A.V. Barsukova Somatic Embryogenesis of Larch and Siberian Pine in Siberia // Forest Science (Lesovedenie). 2012. No 6. С.63-70. Experiments of cultivating the immature zygotic embryos of Siberian coniferous species (Larix sibirica, L.gmelinii, L. sukaczewii, and Pinus sibirica) were performed on the MS, MSG, LV, DCR and AI media with different concentrations of hormones and their proportions. For the induction of embryogenic callus, every species needed the optimized medium supplemented with L-glutamine, casein hydrolyzate, ascorbic acid, and hormones. The active proliferation of the embryonal mass was observed on the same medium with reduced concentration of cytokinins. The somatic embryos initiated from EM matured on the basal medium with ABA and PEG. In spite of the species specificity, the morphogenesis of embryogenic structures had the same scheme: elongation of somatic cells and its asymmetric division and development of globular, torpedo and bipolar somatic embryos. Not all donor-plants of coniferous species could form morphogenic callus and somatic embryos. Four cell lines were found in Larix sukaczewii. The success of the somatic embryogenesis was related to the developmental stage of explant, medium components, hormonal regulation, and tree genotypes. Somatic embryogenesis, nutrient medium, hormones, Larix sibiricа, L. gmelinii, L. sukaczewii, and Pinus sibirica. . |
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