CENTRE ON THE PROBLEMS OF ECOLOGY AND PRODUCTIVITY OF FORESTS
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CENTRE ON THE PROBLEMS OF ECOLOGY AND PRODUCTIVITY OF FORESTS |
RUSSIAN ACADEMY OF SCIENCES |
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I.N. Tretyakova, E.V. Voroshilova Initiation of somatic embryogenesis of Siberian pine megagametophytes in explantation // Forest Science (Lesovedenie). 2014. No 1. С.51-56. The in vitro introduction of Siberian pine megagametophytes in 1/2LV medium with addition of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyl adenine (BAP) as growth regulators is considered. Embriogenic competency of explants was expressed in cellular elongation, coenocytes formation, and migration of nucleus to a cellular pole, fission, and embryoid globules formation. The embryonic-suspensor mass formation occurred. The megagametophytes culture of Siberian pine allows propagation of the unique species. Their biological importance have been foreseen by E.G. Minina. Pinus sibirica, megagametophyte, somatic embryogenesis, embryonic-suspensor mass. . |
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