CENTRE ON THE PROBLEMS OF ECOLOGY AND PRODUCTIVITY OF FORESTS
|
|
CENTRE ON THE PROBLEMS OF ECOLOGY AND PRODUCTIVITY OF FORESTS |
RUSSIAN ACADEMY OF SCIENCES |
|
|
Kontsevaya I.I. Long-term Maintenance of Birch Microplants in Tissue Culture // Forest Science (Lesovedenie). 2009. No 5. P. 50-56 (in Russian). The possibility of maintaining the cultures of downy birch (Betula pubescens Ehrh.), silver birch (B. pendula Roth.) and Karelian birch (B. pendula Roth. var. carelica Merckl.) on nutritional media supplemented with saccharose at higher concentrations, sorbitol, medical charcoal, abscistic acid, and BAP in different combinations during 12 months under in vitro conditions was revealed. Significant differences between the birch species were determined according to their responses to such long-term maintenance. The addition of sorbitol to the nutrient medium at a concentration of 3 g 1 ' after 6 months of maintenance promoted the greater number of viable explants for all the the birch genotypes as compared to those in the control. The above substances did not inhibit the development of the birch explants subcultured on fresh hormone-free media after different periods of maintenance. Long-term maintenance, birch microplants, tissue culture, abscistic acid, sorbitol, medical charcoal |
webmaster |