CENTRE ON THE PROBLEMS OF ECOLOGY AND PRODUCTIVITY OF FORESTS
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CENTRE ON THE PROBLEMS OF ECOLOGY AND PRODUCTIVITY OF FORESTS |
RUSSIAN ACADEMY OF SCIENCES |
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Salyaev R.K., Rekoslavskaya N.I. Production of Tissue Cultures of Siberian Cells // Forest Science (Lesovedenie). 2009. No 5. P. 57-62 (in Russian). Seeds of the Siberian pine, Pinus sibirica Du Tour, taken from superior trees were used for the isolation of embryos that were placed on Murashige and Scoog media in vitro. Conditions for the sterilization of seeds, cotyledons, and hypocotyls of seedlings and the composition of nutrient media to obtain tissue Siberian pine cultures were selected. Pinus sibirica Du Tour, callus culture, tissue culture in vitro, cloning |
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